pET-30a |
Novagen |
5400 |
T7 promoter, T7 terminator |
|
pE194 |
[18] |
3728 |
Erythromycin resistance gene (ermE) |
|
pCD256 |
[43] |
4790 |
Low copy plasmid in L. plantarum
|
|
pCDLbu1 |
[15] |
5776 |
High copy plasmid in L. plantarum
|
|
pCDLbu1_PT7_mCherry_TT7_Ery |
This study |
6425 |
T7 RNA polymerase specific promoter |
|
pCDLbu1ΔEc_PT7_mCherry_TT7_Ery |
This study |
3989 |
T7 RNA polymerase specific promoter, without sequences for replication and selection in E. coli
|
|
pCDLbu1ΔEc_P11_mCherry |
[47] |
3809 |
|
|
pCD256_PlacSynth_mCherry |
This study |
6959 |
Low copy plasmid; promoter PlacSynth; gene of interest mCherry |
IPTG (1 mM) |
pCD256_PlacSynth_T7RNAP |
This study |
8882 |
Low copy plasmid; promoter PlacSynth; gene of interest T7 RNA polymerase |
IPTG (1 mM) |
pCDLbu1_PlacSynth_mCherry |
This study |
8164 |
High copy plasmid; promoter PlacSynth; gene of interest mCherry |
IPTG (1 mM), TMG (17 mM) |
pCDLbu1_PlacA_mCherry |
This study |
7917 |
High copy plasmid; promoter PlacA; gene of interest mCherry |
Lactose (0.5 -2 % w/v) |
pCDLbu1_PxylA_mCherry |
This study |
8077 |
High copy plasmid; promoter PxylA; gene of interest mCherry |
Xylose (0.2 -2 % w/v) |
pCDLbu1_PxylA(nativeRBS)_mCherry |
This study |
8077 |
High copy plasmid; promoter PxylA; gene of interest mCherry; native RBS and spacer sequence from B. megaterium DSMZ 319 xylA
|
Xylose (2 % w/v) |
Strains |
|
|
|
|
B. megaterium DSMZ 319 |
DSMZ |
|
|
|
E. coli Neb10β |
NEB |
|
|
|
L. plantarum CD033 |
[43] |
|
|
|
L. plantarum 3NSH |
[17] |
|
Plasmid cured L. plantarum CD033 |
|