Table 2.
Plasmids and strains used in this study
| Plasmid | Reference | Size (bp) | Relevant characteristics | |
|---|---|---|---|---|
| pET-30a | Novagen | 5400 | T7 promoter, T7 terminator | |
| pE194 | [18] | 3728 | Erythromycin resistance gene (ermE) | |
| pCD256 | [43] | 4790 | Low copy plasmid in L. plantarum | |
| pCDLbu1 | [15] | 5776 | High copy plasmid in L. plantarum | |
| pCDLbu1_PT7_mCherry_TT7_Ery | This study | 6425 | T7 RNA polymerase specific promoter | |
| pCDLbu1ΔEc_PT7_mCherry_TT7_Ery | This study | 3989 | T7 RNA polymerase specific promoter, without sequences for replication and selection in E. coli | |
| pCDLbu1ΔEc_P11_mCherry | [47] | 3809 | ||
| pCD256_PlacSynth_mCherry | This study | 6959 | Low copy plasmid; promoter PlacSynth; gene of interest mCherry | IPTG (1 mM) |
| pCD256_PlacSynth_T7RNAP | This study | 8882 | Low copy plasmid; promoter PlacSynth; gene of interest T7 RNA polymerase | IPTG (1 mM) |
| pCDLbu1_PlacSynth_mCherry | This study | 8164 | High copy plasmid; promoter PlacSynth; gene of interest mCherry | IPTG (1 mM), TMG (17 mM) |
| pCDLbu1_PlacA_mCherry | This study | 7917 | High copy plasmid; promoter PlacA; gene of interest mCherry | Lactose (0.5 -2 % w/v) |
| pCDLbu1_PxylA_mCherry | This study | 8077 | High copy plasmid; promoter PxylA; gene of interest mCherry | Xylose (0.2 -2 % w/v) |
| pCDLbu1_PxylA(nativeRBS)_mCherry | This study | 8077 | High copy plasmid; promoter PxylA; gene of interest mCherry; native RBS and spacer sequence from B. megaterium DSMZ 319 xylA | Xylose (2 % w/v) |
| Strains | ||||
| B. megaterium DSMZ 319 | DSMZ | |||
| E. coli Neb10β | NEB | |||
| L. plantarum CD033 | [43] | |||
| L. plantarum 3NSH | [17] | Plasmid cured L. plantarum CD033 |