Figure 1.
Early Morphological Patterns of Developing Colonies
(A) Fluorescence images of typical Y-shaped structures observed at t = 3–4 hr during colony formation by AR16 (PrrnE-gfp) cells.
(B) Fluorescence images of linear (1, 1′) and multi-branched (2, 2′) structures, observed at t = 2.5 hr (1, 2) and subsequently at t = 4 hr (1′, 2′) during colony formation by AR16 cells.
(C) Fluorescence images of initial chain branching and Y-shape formation of AR16 developing colony at the indicated time points. White arrow points to the position of chain breakage, and yellow and red arrows indicate single- and double-chained Y arms, respectively.
(D) Frequency of each of the initial morphologies described above (A and B), as calculated from 11 separate experiments. The total colony number was 272.
Scale bars represent 5 μm.