Figure 2.
Characterizing Stages of Colony Development
(A) Time-lapse fluorescence images of AR16 (PrrnE-gfp) developing colony at the defined stages and the indicated time points. Each stage was pseudo-colored and overlaid over previous stages. Color map corresponds to the different stages. Scale bars represent 40 μm.
(B and C) Cells (PY79) forming a Y shape were irradiated at the indicated position of the double-chained Y arm (blue frame). The irradiated cells were marked by the addition of PI (red), and their location (highlighted by arrows) was tracked at the indicated time points. Shown are overlaid transmitted light and fluorescence images. Representative experiments out of 13 independent biological repeats. Scale bars represent 20 μm.