Figure 3.

Dimerization at the Smc Hinge Hinders Chromosomal Association of Smc/ScpAB

(A) ChIP-qPCR was performed on exponentially growing cells of strains BSG1051–BSG1052, BSG1406, and BSG1387 using α-Smc antiserum. Quantification of input and eluate material was done by qPCR using primer pairs specific for the indicated loci.

(B) As in (A) using strains BSG1889–BSG1894.

(C) The scheme indicates the disruptive effect of mutations in the Smc hinge domain on dimerization. ChIP-qPCR was performed with α-Smc antiserum on strains BSG1620–BSG1621, BSG1624, BSG1890, and BSG1892–BSG1893.

(D) Fluorescence imaging of Smc-mGFP fusion proteins in cells of strains BSG1067–BSG1068, BSG1378, BSG1413, BSG1662, BSG1677, and BSG1798–BSG1799. Scale bar, 2 μm. Quantification of foci number per cell is given in Figure S4C. Same experiments as in Figure 1C.

See also Figure S3.