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. Author manuscript; available in PMC: 2017 Feb 25.
Published in final edited form as: Cell. 2016 Feb 25;164(5):974–984. doi: 10.1016/j.cell.2016.02.008

Figure 2. Untrimmed piRNAs are loaded onto PRG-1 and possess 2′–O–methylation.

Figure 2

(A) Bar plots showing the change in small RNA reads matching indicated genome annotations between input and PRG-1 IP samples prepared from wild type and parn-1 strains.

(B) Correlation analysis of the piRNA level. Libraries were prepared from PRG-1 IP samples from WT and parn-1 strains. Data from reads for each piRNA were normalized to total reads in the same sample. For a perfect correlation, the Spearman rank correlation coefficient (r) = 1 or −1, and for no correlation, r = 0.

(C) Oxidation and β–elimination followed by Northern blot analysis of RNA prepared from WT, parn-1 and henn-1 strains. RNA samples were not treated (−) or β–elimination treated (+), and probed for 21UR-1949. Probing for miRNA-66 served as controls for loading and β–elimination reactions.

(D) Fold enrichment of piRNA reads was calculated by comparing β–eliminated and non-treated samples. Small RNA libraries were generated from β–eliminated and untreated RNAs prepared from WT, parn-1, henn-1 strains.

See also Figure S2.