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. Author manuscript; available in PMC: 2017 Feb 25.
Published in final edited form as: Cell. 2016 Feb 25;164(5):974–984. doi: 10.1016/j.cell.2016.02.008

Figure 5. PARN-1 is required for fertility and epigenetic silencing of single-copy transgenes.

Figure 5

(A) Brood size counts in WT, prg-1, parn-1, prg-1; parn-1 double, and parn-1 rescue animals at 25 °C. n=Number of parental adults scored.

(B, C) Schematics of the gfp::cdk-1 reporter (B), and of the h2b::gfp::21U-RNA-target-site reporter (C), that were injected into wild-type, parn-1 and prg-1 worms to establish single copy transgenic lines. Images of GFP fluorescence signals in the resulting strains are shown. Oocyte nuclei that either express, or fail to express the construct are indicated by arrowheads. The dashed lines outline the position of germline. Bright signals outside of the germline are from gut granule autofluorescence. The gfp::h2b::21U-RNA-target-site reporter established in wild-type and parn-1 animals was crossed to the rde-3 mutant strain. n=number of independent transgenic lines exhibiting the pattern of expression shown.