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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Integr Biol (Camb). 2015 Jul 20;7(9):1068–1078. doi: 10.1039/c5ib00151j

Fig. 2. Spatially-defined patterns of localized cell groups on mesentery tissue and single-cell printing capability.

Fig. 2

(A) 4×4 array pattern (center-to-center spacing, dspacing = 0.8 mm) of circular 'spots' of CellTracker Red (CTR)-labeled MDA-MB-231 cancer cells on vascularized mesentery tissue, imaged two hours following LDW. Mesenteric arterioles (A), venules (V), and lymphatic vessels (L) were readily identifiable by lectin labeling. (B) Contiguous 4×4 array of MDA-MB-231 cancer cells generated by reducing dspacing to 0.4 mm. (C) 4×4 array (dspacing = 0.8 mm) of nearly-single cancer cell groups. (D) Phase contrast images of trypsinized MDA-MB-231 cells on the print ribbon at various loading concentrations (black text) and corresponding areal density (white text). Circle borders indicated cell groups selected for printing under typical transfer area.

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