Fig. 2. Spatially-defined patterns of localized cell groups on mesentery tissue and single-cell printing capability.
(A) 4×4 array pattern (center-to-center spacing, dspacing = 0.8 mm) of circular 'spots' of CellTracker Red (CTR)-labeled MDA-MB-231 cancer cells on vascularized mesentery tissue, imaged two hours following LDW. Mesenteric arterioles (A), venules (V), and lymphatic vessels (L) were readily identifiable by lectin labeling. (B) Contiguous 4×4 array of MDA-MB-231 cancer cells generated by reducing dspacing to 0.4 mm. (C) 4×4 array (dspacing = 0.8 mm) of nearly-single cancer cell groups. (D) Phase contrast images of trypsinized MDA-MB-231 cells on the print ribbon at various loading concentrations (black text) and corresponding areal density (white text). Circle borders indicated cell groups selected for printing under typical transfer area.