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. Author manuscript; available in PMC: 2016 Mar 10.
Published in final edited form as: Proteomics. 2009 May;9(10):2888–2891. doi: 10.1002/pmic.200800873

Figure 2.

Figure 2

Multiple uses of LAP-tagged stable cell lines. (a) Avoidance of toxic effects associated with over-expression of cell cycle regulatory proteins, such as Emi1, by tetracycline-regulated transient protein expression. Tandem affinity purification of LAP-Emi1, identifies its interaction with the Anaphase Promoting Complex/Cyclosome (APC/C). For a and c, MW= molecular weight, In= input, El= eluate, for peptide information see Supplementary Table. 1. (b) Protein sub-cellular localization of KATNAL1, a novel microtubule-associated protein, via in vivo time-lapse fluorescence microscopy and post fixation immunofluorescence. Bar = 10 μm. (c) Purification of LAP-KATNAL1 and identification of interacting proteins by mass spectrometry. (d) Other uses: RNAi-coupled rescue experiments and TAP (iTAP), cell cycle specific expression, and over-expression.