. 2004 Jul 2;101(28):10398–10403. doi: 10.1073/pnas.0403342101

Table 1. Lymphadenopathy in recipients of B7-deficient CD4⁺ T cells.

Donor CD4 cells	LN cells,^*n × 10⁶	CD4 cells, %	CD4 cells, n × 10⁶	Spleen cells,^*n × 10⁶	CD4 cells, %	CD4 cells, n × 10⁶
B7^-/-	4.0 ± 0.85	51.5	2.0 ± 0.44	24.0 ± 3.39	21.5	5.16 ± 0.73
B7^+/+	0.4 ± 0.04	26.8	0.1 ± 0.01	27.6 ± 3.20	16.7	4.6 ± 0.53

CD4⁺ T cells purified (98% pure) from B7 wt (+/+) or B7-deficient (-/-) donor mice were transferred intravenously into syngeneic RAG-2-deficient recipients. Recipients of unseparated CD4⁺ T cells were killed 30 days after transfer. The mandibular, superficial cervical, axillary, superficial inguinal and mesenteric lymph nodes (LNs), and spleens of all recipients were isolated, and the total cell numbers were determined. The percentage of CD4⁺ T cells in lymph nodes and spleens were determined by FACS analysis by using anti-CD4 (Pharmingen RM4-5 PE) on a Beckman—Coulter. Five mice were analyzed per group. Data are given as mean ± SD.

Mandibular, cervical, axillary, inguinal, and mesenteric lymph nodes (LN).

Table 1. Lymphadenopathy in recipients of B7-deficient CD4+ T cells.

Table 1. Lymphadenopathy in recipients of B7-deficient CD4⁺ T cells.