Table 2. Gastritis/colitis in mice given CD4 cells deficient in B7 expression.
| B7 genotype of donor CD4+T cells
|
Mean gastritis score (grade 0.0-3.0)
|
Severe gastritis (grade 2.5-3.0)
|
Mean colitis score (grade 0.0-5.0)
|
Severe colitis (grade 3.0-5.0)
|
|
|---|---|---|---|---|---|
| CD4CD25- | CD4CD25+ | ||||
| -/- | None | 2.5 ± 0.8 | 6/8* | 4.0 ± 1.3 | 6/8 |
| -/- | -/- | 2.6 ± 0.4 | 4/5 | 4.2 ± 0.8 | 4/5 |
| -/- | +/+ | 2.3 ± 0.5 | 6/8* | 3.5 ± 1.5 | 6/8 |
| +/+ | None | 1.7 ± 1.2 | 3/5 | 4.7 ± 0.6 | 5/5 |
| +/+ | -/- | Not done | Not done | Not done | Not done |
| +/+ | +/+ | 0.0 + 0.0 | 0/5 | 0.0 + 0.0 | 0/5 |
Th (CD4CD25-) or T-reg (CD4CD25+) cells (n = 106) purified (98% pure) from B7 wt or B7-deficient donor mice were transferred i.v. into syngeneic RAG-2-deficient recipients. Recipients were killed at day 8. All mice were preserved in Bouins' solution. Histological analyses of skin, stomach, and colon and disease scoring were performed by the Harvard University Rodent Pathology Core. Five to 10 mice were analyzed per group.
*
n = 10. Two mice died of wasting before histological analysis.