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. Author manuscript; available in PMC: 2017 Mar 7.
Published in final edited form as: Dev Cell. 2016 Mar 7;36(5):562–571. doi: 10.1016/j.devcel.2016.02.010

Fig. 4. Expanded expression of Pumilio blocks germ cell differentiation.

Fig. 4

(A) Schematic of the UAS-full length pumilio transgenes that carry endogenous pumilio 3′UTR (pum-pum) or α-Tubulin 3′UTR (pum-Tub). (B) Quantification of the percent of egg chambers with polyploid nurse cells or germ cell tumors upon expression of the pum-pum or pum-Tub transgenes driven by germline specific vasa-gal4 driver (n= >100 egg chambers). (C) vasa-gal4 alone (control), (D) vasa-gal4>UAS-pum-pum 3′UTR (vasa-gal4>pum-pum) and (E) vasa-gal4>UAS-pum-Tub 3′UTR (vasa-gal4>pum-Tub) germaria stained for Hts (green) and Vasa (Vas) (magenta). (F) vasa-gal4 alone (control), (G) vasa-gal4>pum-pum 3′UTR and (H) vasa-gal4>pum-Tub 3′UTR germaria stained for Sxl (green) and Rbfox1 (magenta). Scale bars represent 10 μm.