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. Author manuscript; available in PMC: 2016 Mar 10.
Published in final edited form as: Cancer Lett. 2014 Nov 20;357(1):231–241. doi: 10.1016/j.canlet.2014.11.036

Fig. 4.

Fig. 4

The effects of genistein on the sensitivity of MTX and HSC70 expression in L1210/0 and L1210/DDP cells. (A) Cell survival of L1210/0 and L1210/DDP cells after being treated with 0.01 μM MTX for 48 h ± 5 μM genistein for 6 h. MTX significantly inhibited the cell survival in L1210/0 cells when compared to L1210/DDP cells, and genistein enhanced the resistance to MTX in L1210/0 cells. The bar graph represents three independent experiments. Student t-test was used in statistical analysis and * indicates p < 0.05. (B) HSC70 expression in L1210/0 and L1210/DDP cells after treatment with 50 μM or 300 μM of genistein for 6 h. The cell lysates were collected and HSC70 was detected by Western blotting assay. Beta-actin was used as the loading control. The blots shown are a representation of three independent experiments.