Fig. 4.

Identification of an HRE in the CD18 promoter. (A) Depicted is the HRE within the CD18 gene promoter. The core motif 5′-ACGTG-3′ spans nucleotides +1to +5, and the ancillary sequence 5′-CAGAC-3′ spans nucleotides +14 to +18. (B) Chromatin immunoprecipitation was used to examine HIF-1α binding to the CD18 promoter in normoxic and hypoxic U937 cells. Reaction controls included immunoprecipitations performed by using a nonspecific IgG monoclonal antibody (Cntl IP) and PCR performed by using whole leukocyte genomic DNA (Input). A representative example of three independent experiments is shown.