Fig. 3.

REST/NRSF is selectively recruited to target genes. (a) EMSA analysis of U373 protein extracts incubated with a radiolabeled RE1 derived from the NaV1.2 gene shows a specific “shift” (*) that can be supershifted in the presence of an anti-REST antibody (**), confirming the presence of functional REST protein within U373 cell nuclei. (b) ChIP analysis carried out on U373 cells by using an anti-REST antibody shows detectable levels of U373 REST occupancy only at the RE1s of the SNAP25 and L1CAM genes.