. 2016 Jan;12(1):20150708. doi: 10.1098/rsbl.2015.0708

Table 1.

Contractile kinetics under normoxia and hypoxia in ventricular muscle preparations from European sea bass fish in a given condition. Control saline contained 1 nM adrenaline, SR inhibition was achieved with 10 µM ryanodine and 2 µM thapsigargin and high adrenaline contained 1 µM adrenaline. In total, 0.8 Hz is representative of the in vivo heart rate of sea bass at 12°C and bradycardia (0.2 Hz) occurs during hypoxia. n = 5 in all cases except where marked with a dagger, in which n = 4. Bold typeface and asterisks denote a significant difference between hypoxia-tolerant fish and hypoxia-sensitive fish in a given condition (GLM).

	control				SR inhibition				high adrenaline
	hypoxia-tolerant		hypoxia-sensitive		hypoxia-tolerant		hypoxia-sensitive		hypoxia-tolerant		hypoxia-sensitive fish
normoxia	0.2 Hz	0.8 Hz	0.2 Hz	0.8 Hz	0.2 Hz	0.8 Hz	0.2 Hz	0.8 Hz	0.2 Hz	0.8 Hz	0.2 Hz	0.8 Hz
rate of contraction (mN mm⁻² s⁻¹)	5.71	6.43	3.21	3.23	5.74	7.48	2.83	3.42	10.17	11.88	5.38	5.84
rate of contraction (mN mm⁻² s⁻¹)	(±1.39)	(±1.78)	(±0.64)	(±0.80)	(±1.21)	(±1.48)	(±0.70)	(±0.74)	(±2.18)	(±2.50)	(±1.00)	(±1.01)
rate of relaxation (mN mm⁻² s⁻¹)	6.35	6.63	3.49	3.08	6.31	7.34	3.05	3.15	11.37	10.30	6.47	5.85
rate of relaxation (mN mm⁻² s⁻¹)	(±1.72)	(±1.99)	(±0.60)	(±0.83)	(±1.53)	(±1.56)	(±0.66)	(±0.68)	(±2.70)	(±2.40)	(±1.03)	(±0.98)
resting tension (mN mm⁻²)	2.45	2.37	1.05	1.02	2.23	2.28	0.79	0.79	2.24	2.27	0.63	0.64
resting tension (mN mm⁻²)	(±0.85)	(±0.83)	(±0.49)	(±0.47)	(±0.84)	(±0.85)	(±0.38)	(±0.36)	(±0.85)	(±0.86)	(±0.36)	(±0.36)
hypoxia	0.2 Hz	0.8 Hz	0.2 Hz	0.8 Hz	0.2 Hz	0.8 Hz	0.2 Hz	0.8 Hz	0.2 Hz	0.8 Hz^†	0.2 Hz	0.8 Hz^†
rate of contraction (mN mm⁻² s⁻¹)	8.59	7.78	2.16	2.68	8.89	8.97	1.71	2.15	12.19	9.24	4.22	3.38
rate of contraction (mN mm⁻² s⁻¹)	(±1.76)	(±1.08)	(±0.47)*	(±0.34)*	(±2.14)	(±1.57)	(±0.33)*	(±0.42)*	(±2.29)	(±1.48)	(±0.97)*	(±1.05)
rate of relaxation (mN mm⁻² s⁻¹)	10.54	7.98	2.77	2.71	10.24	14.88	1.59	2.09	14.88	8.57	5.49	3.44
rate of relaxation (mN mm⁻² s⁻¹)	(±1.85)	(±0.95)	(±0.64)*	(±0.51)*	(±2.49)	(±2.54)	(±0.34)*	(±0.40)*	(±2.54)	(±1.42)	(±1.44)*	(±1.35)
resting tension (mN mm⁻²)	1.74	1.77	0.37	0.38	1.41	1.44	0.29	0.35	1.31	1.39	0.34	0.06
resting tension (mN mm⁻²)	(±0.88)	(±0.90)	(±0.36)	(±0.37)	(±0.83)	(±0.82)	(±0.40)	(±0.41)	(±0.79)	(±1.08)	(±0.37)	(±0.39)