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Effects of partially purified rat hypothalamic fractions on the internalization of the rat apelin receptor-EGFP. Confocal images of rat apelin receptor-EGFP CHO cells. Before ligand exposure (T0) or in presence of the inactive apelin fragment R10F (100 nM), the apelin receptor is confined to the plasma membrane. Internalization of the apelin receptor occurs in presence of pE13F (100 nM) or the rat hypothalamic fractions 35, 36, or 37.
