Table 1. The affinity of Galmic and galnon for galanin receptors.
Ki, μM
|
||
---|---|---|
Ligand | Bowes cells (hGalR1) | CHO cells (rGalR2) |
Galmic | 34.2 | >100 |
Galnon | 11.7 | 34.1 |
Ki values of Galmic and galnon at GalR1 and GalR2 receptors were determined by displacement of 125I-galanin from membranes prepared from human Bowes cells (hGalR1) and stably transfected Chinese hamster ovary (CHO) cells expressing rat GalR2, respectively. 125I-galanin concentration was 0.2 nM, and Galmic and galnon concentrations were between 10-8 M and 10-4 M. Ki values were calculated with prism software.