Table 2. Trichome phenotype, length of actively dividing cells, and effects of exogenous auxin on lateral root growth.
Measurement | WT | mya2-1 |
---|---|---|
Unbranched trichomes in percent of total trichome no. at cauline leaves | 7.2 ± 3.1 | 48 ± 5.2 |
Trichome length, mm | 0.94 ± 0.05 | 1.10 ± 0.04 |
Meristematic cell length in actively dividing cell files of root tips, μm | 4.2 ± 0.26 | 5.5 ± 0.24 |
Primary root length of vertically grown 5-day-old seedlings, mm | 15.2 ± 0.4 | 7.9 ± 0.3 |
No. of lateral roots per cm of root length of 11-day-old seedlings | 1.5 ± 0.15 | 0.5 ± 0.20 |
No. of lateral roots per cm of root length after addition of auxin* | 2.5 ± 0.7 | 3.2 ± 0.4 |
Lateral root length of seedlings after addition of auxin, mm* | 1.9 ± 0.12 | 3.4 ± 0.93 |
Values shown are ± SE. All mutant values are significantly different (P < 0.05) from controls.
To test for effects of exogenous auxin on root growth, lateral roots were measured in 12-day-old seedlings that had been transferred to plates containing 10 μm auxin after 5 days of growing vertically on Murashige and Skoog medium agar.