Table 1.
Proteins showing consistent increase, or decrease, in abundance with increasing Nil concentration. Gels similar to those shown in the Figure 3 were subjected to image analysis to identify differentially expressed proteins. These spots were then cut from the gels and proteins were identified via tandem mass spectrometry (MS). Cross correlation (XC) score is a metric associated with the quality of identification, with a score >20 indicating a positive identification; pI/MW is isoelectric point and molecular weight; Cov (%) is the fraction of protein sequence coverage identified via MS; Acce.No. is accession number for the protein.
| Spot ID | Protein name/Gene name | Functional category | XC score | pl/MW | Cov (%) | Acce. No. |
|---|---|---|---|---|---|---|
| Proteins with increased abundance | ||||||
| 607 | Immuno dominant antigen A/IsaA | Catalysis of the hydrolysis of any glycosyl bond | 60 | 5.9/24 | 26 | NP_373093 |
| 703 | Major cold shock protein/cspA | Transcription | 50 | 4.4/7 | 40 | NP_371926 |
| 740 | Hypothetical protein/SAV1875 | Putative intracellular protease/amidase | 100 | 4.4/19 | 60 | NP_372399 |
| 779 | Aldehyde dehydrogenase/SAV2122 | Energy production and conversion | 318 | 4.9/52 | 57 | NP_372646 |
| 818 | General stress protein 20U/dps | DNA-binding ferritin like protein | 128 | 4.4/17 | 72 | NP_372663 |
| 910 | Phosphoglycerate kinase/pgk | Carbohydrate transport and metabolism | 304 | 5.0/43 | 60 | NP_371297 |
| 912 | ABC transporter ATP-binding protein/SAV0842 | Post-translational modification, protein turnover | 216 | 4.7/28 | 76 | NP_371366 |
| 955 | 3-oxoacyl-(acyl-carrier protein) reductase/fabG | Fatty acid biosynthesis | 220 | 5.5/26 | 69 | NP_371755 |
| 967 | Hypothetical protein/SAV1067 | Nucleotide transport and metabolism | 120 | 4.6/10 | 98 | NP_371591 |
| 1014 | Universal stress protein family/SAV1710 | Cellular processes and signalling | 316 | 5.5/18 | 90 | NP_372234 |
| 1019 | Hypothetical protein/SAV2581 | Predicted hydrolases or acetyltranferases | 300 | 4.6/31 | 67 | NP_373105 |
| 1061 | Hypothetical protein/SAV1015 | Information storgae and processing | 188 | 4.7/19 | 65 | NP_371539 |
| Proteins with reduced abundance | ||||||
| 152 | Universal stress protein(USP)/SAV1710 | Cellular processes and signalling | 80 | 5.5/19 | 60 | NP_372234 |
| 153 | Alkaline shock protein/asp23 | Alkaline pH tolerance | 134 | 4.9/19 | 56 | NP_372706 |
| 248 | Cysteine synthase/cysK | Aminoacid and transport and metabolism | 138 | 5.2/33 | 49 | NP_371037 |
| 535 | Ribosomal subunit interface protein/RaiA | A stress response protein | 100 | 5.0/22 | 41 | NP_371276 |
| 739 | Catalase/katA | ubiquitous enzyme protect cells from toxic effects | 164 | 5.2/58 | 35 | NP_646038 |
| 814 | Ornithine-oxo acid transaminase/rocD | Aminoacid and transport and metabolism | 298 | 5.1/43 | 58 | NP_371481 |
| 868 | Aldehyde dehydrogenase/SAV1875 | Energy production and conversion | 216 | 4.9/52 | 52 | NP_372646 |
| 926 | Alcohol dehydrogenase/adh1 | Catalysis of rection related to alcohol to produce energy | 320 | 5.2/36 | 65 | NP_645385 |
| 941 | 2-amino-3-keto butyrate coenzyme A ligase/MW0505 | Glycine, serine and threonine metabolism | 198 | 5.1/43 | 46 | NP_645322 |
| 942 | Coenzyme A disulfide reductase/cdr | Catalyzes specifically the NADPH-dependent reduction | 138 | 5.2/49 | 38 | O52582 |