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. 2016 Mar 10;11(3):e0150582. doi: 10.1371/journal.pone.0150582

Fig 7. RT-qPCR validation of the differentially expressed genes (DEGs) in response to SMV infections with three isolates.

Fig 7

Based on the results from RNA-seq combined with sRNA-seq and degradome-seq analysis, 8 DEGs that supposedly represented the majority of DEGs in response to SMV infection at 14 dpi were selected for validation by RT-qPCR analysis. The soybean Actin (GmACT11) gene was used as an internal control. Error bars represent mean ± SD (standard deviation) and the data are averages from three biological replicates. Asterisks indicate statistically significant differences comparing with the mock control (student’s t-tests) (*p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant).