Fig 3. Inoculated HT-5637 urothelial cells produce extracellular vesicles (EV) with epigenetic potential.

(A) Unlabelled EV from Fim H⁺ inoculated host cells (vs from mock-infected cells) were able to upregulate epigenetic machinery (EZH2) in naïve urothelial (HT-5637) cells, (p<0.005). (B) EZH2 fluorescent signals were imaged by confocal microscopy and quantitated using Volocity software. (C) Quantitation of nuclear size was performed using Volocity software, based on the area occupying the Hoechst 33342 signal. Fim H⁺ -inoculated host cells had smaller nuclei than control groups. N = 6.