Table 5. MICs and MBCs of sixteen antimicrobial SecA inhibitors against bacteria (μg/mL).

Compoundsx	L. crescens		R. etli		B. japonicum		M. loti		S. meliloti		X. citri subsp. citri		A. tumefaciens		E. coli
	MIC	MBC	MICy	MBCz	MIC	MBC	MIC	MBC	MIC	MBC	MIC	MBC	MIC	MBC	MIC	MBC
C16	32	32	32	64	32	64	16	32	16	32	16	32	64	64	256	512
C17	16	16	32	32	16	16	16	32	32	64	16	32	32	128	512	512
C18	16	16	32	64	64	64	16	32	32	64	32	64	32	128	512	512
C19	32	32	32	64	16	64	8	16	16	16	16	32	32	128	256	512
C20	16	32	32	32	32	32	8	16	32	64	16	32	64	128	512	512
SA1	32	32	32	64	16	32	16	32	16	32	32	64	32	64	512	512
SA2	32	32	32	64	64	128	16	32	16	32	32	64	32	64	512	512
SA3	32	32	32	64	32	32	16	32	32	64	32	64	32	128	512	512
SA4	32	32	32	64	32	32	16	32	16	32	64	128	32	64	512	512
SA5	16	32	64	64	32	64	16	32	32	64	64	128	32	64	512	512
SA6	32	64	16	32	16	16	16	32	32	64	32	64	16	64	512	512
SA7	16	32	32	32	32	32	16	32	32	64	64	128	32	64	256	512
SA8	32	64	64	64	32	32	16	32	32	32	64	128	16	64	256	512
SA9	32	32	32	32	32	32	16	32	16	32	32	64	16	64	512	512
SA10	32	32	16	32	32	32	16	32	16	32	32	64	32	128	256	512
SA11	32	64	16	32	32	64	16	32	16	32	32	64	32	64	512	512
STM	32	32	16	16	32	32	16	32	64	128	32	32	64	128	128	256
MMXw	0.26	>0.5	0.26	>0.5	0.26	>0.5	>0.5	>0.5	>0.5	>0.5	>0.5	>0.5	>0.5	>0.5	>0.5	>0.5

^w A 2-fold dilution series of MMX without SecA inhibitors ranged from 1.024 to 0.008% (V/V).

^x A 2-fold dilution series ranging from 1024 to 8 μg/mL of SecA inhibitors were prepared in medium broth, in which the initial concentration of MMX was 1.024% (V/V).

^y MIC is the lowest concentration that inhibits visible growth of a microorganism after incubation.

^z MBC is defined as the lowest concentration with the reduction by 99.9% of the initial inoculum of multiplying bacteria.