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. 2016 Feb 25;1(2):e85096. doi: 10.1172/jci.insight.85096

Figure 2. Collecting lymphatic vessel function in the lower limbs.

Figure 2

(AD) Near-infrared fluorescence imaging of the normal lower limb lymphatic network in WT mice (A) and three representative images of the phenotype seen in Prox1+/– mice (BD). Three phenotypes are consistently observed in all Prox1 heterozygous mice: dermal backflow (brackets), collateral rerouting (white arrow), and interstitial leakage (arrowheads). All the pictures are oriented in the same direction, where P is proximal and D is distal to the popliteal lymph node. Red arrows indicate the direction of the flow. (E and F) Collecting vessel contractility in young (E) and old (F) WT and Prox1+/– mice. No difference was seen between young WT and Prox1+/– mice, but contractility was significantly reduced in old Prox1+/– mice. (G and H) Response to mechanostimulation in young (G) and old (H) WT and Prox1+/– mice. Young and old Prox1+/– mice had less efficient uptake into deep collectors after mechanostimulation. MFI, mean fluorescence intensity. **P ≤ 0.01; ***P ≤ 0.0001, 2-tailed Student’s t test. Scale bar: 2,000 μm.