A, Genetic organization of the mba genes located at the minor chromosome (C2). Genes are represented with block arrows. Sizes of the genes and the intergenic regions are on scale. The transcriptional promoters are represented with black arrows oriented in the transcription sense. B, Genetic organization of the mba (orb) genes in Burkholderiales strains. The mba (orb) sequences (accession number) are B. xenovorans LB400 (BxeB0517–BxeB0531), B. phytofirmans PsJN (Bphyt_4063–Bphyt_4079), C. fungivorans Ter331 (CFU_2304–CFU_2292), B. pseudomallei K96243 (mbaF–mbaE), B. pseudomallei 668 (BURPS6681917–BURPS668 1931), B. pseudomallei MSHR346 (GBP346_A1956–GBP346_A1970), B. vietnamiensis G4 (Bcep1808 1577–Bcep1808 1590), Burkholderia sp. KJ006 (MYA_1458–MYA_1471), B. cenocepacia J2315 (orbS–orbL), B. cenocepacia PC184 (BCPG00175–BCPG00162) and B. phymatum STM815 (Bphy4047–Bphy4035). The gene organization of B. pseudomallei K96243 is shared by B. mallei SAVP1 (BMASAVP1A_1634–BMASAVP1A _1621), B. mallei NCTC 10229 (BMA10229_A0295–BMA10229_A0284), B. mallei NCTC 10247 (BMA10247_0864–BMA10247_0877), B. thailandesis E264 (BTH I2427–BTH I2414) and B. pseudomallei 1710b (BURPS1710b_2076–BURPS1710b_2092). The gene organization of B. pseudomallei 668 is shared by B. pseudomallei 1106a (BURPS1106A 1931–BURPS1106A 1917) and B. pseudomallei BPC006 (BPC006_I1984–BPC006_I1999). The gene organization of B. cenocepacia J2315 is shared by B. ambifaria MC40-6 (BamMC406_1550–BamMC406_1564), B. cepacia AMMD (Bamb 1529–Bamb 1543), B. cenocepacia AU1054 (Bcen 1152–Bcen 1166), B. cenocepacia HI2424 (Bcen2424 1632–Bcen2424 1646), Burkholderia sp. 383 (Bcep18194 A4778–Bcep18194 A4792), B. multivorans ATCC17616 (Bmul 1606–Bmul 1593) and B. cepacia GG4 (GEM_1784–GEM_1770).