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. 2016 Mar 10;11(3):e0151273. doi: 10.1371/journal.pone.0151273

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© 2016 Vargas-Straube et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — The dendogram of MbaE formyltransferase from B. xenovorans LB400 and other formyltransferases was constructed by the neighbor-joining method using MEGA 6.0 based on multiple sequence alignment by Clustal W. Sequence of deduced protein from mbaE gene from B. xenovorans LB400 is highlighted (black circle). One clade includes the L-N^δ-hydroxy- ornithine formyltransferases (accession number) that may produce L-N^δ-hydroxy-N^δ-formylornithine and L-N^δ-hydroxy-N^α-formylornithine: MbaE B. xenovorans LB400 (YP554769.1), MbaE B. phytofirmans PsJN (YP001887830.1), MbaE C. fungivorans Ter331 (YP004752942.1), MbaE B. pseudomallei 668 (YP001058966.1), MbaE B. pseudomallei 1106a (YP001066211.1), MbaE B. pseudomallei 1710b (YP333487.1), MbaE B. pseudomallei K96243 (YP108374.1), MbaE B. pseudomallei MSHR345 (YP002896675.1), MbaE B. mallei SAVP1 (YP992947.1), MbaE B. mallei NCTC 10229 (YP001026286.1), MbaE B. mallei NCTC 10247 (YP001080440.1), MbaE B. pseudomallei BPC006 (YP006652779.1) and MbaE B. thailandesis E264 (YP442935.1). The other clade includes L-N^δ-hydroxy-ornithine formyltransferases (accession number) that produce L-N^δ-hydroxy-N^δ-formylornithine: PvdF P. aeruginosa PA01 (NP251086.1), PvdF B. vietnamientis G4 (YP001119431.1), PvdF Burkholderia sp. KJ006 (YP006332563.1), PvdF Burkholderia sp. 383 (YP369030.1), PvdF B. cenocepacia AU1054 (YP621045.1), PvdF B. cenocepacia HI2424 (YP835290.1), PvdF B. cenocepacia PC184 (YP002091473.1), PvdF B. cenocepacia J2315 (YP002230827.1), PvdF B. multivorans ATCC17616 (YP001579778.1), PvdF B. cepacia GG4 (YP006615890.1), PvdF B. ambifaria MC40-6 (YP001808266.1), PvdF B. cepacia AMDD (YP773434.1). The methionyl-tRNA_f^Met formyltransferases (accession number) are Fmt B. xenovorans LB400 (YP556637.1) and Fmt E. coli K-12 (YP492145.1) and the glycinamide ribonucleotide formyltransferases (accession number) are PurN B. xenovorans LB400 (YP560177.1) and PurN E. coli K-12 (YP490728.1).