Fig 3. Genetic interactions between cycG and Delta mutants during wing development.

(A) Homozygous cycG^HR7 mutants have wild type looking wing veins, labelled as in Fig 2. The 12 positions used for phenotypic evaluation are framed. Vein thickening at any of these positions was valued 1, wild type was valued 0. (B) Wings of heterozygous Dl^B2 / + flies display thickened veins and deltas at the margin; arrows point to some examples. Male wing; the phenotype is generally slightly stronger in females than in males (see D). (C) Wings of the combined Dl^B2 cycG^HR7 / + cycG^HR7 flies display an enhanced vein thickening phenotype (female; arrows point to some examples). (D) Numerical evaluation according to (A) of wings from Dl^B2 / + (1, darker colours) and Dl^B2 cycG^HR7 / + cycG^HR7 (2, lighter colours) flies. Females (f) in reddish, males (m) in bluish colours; (n = 10). The Y-axis represents the values of positions with phenotypic aberration per wing (pp). Statistical significance was determined by Student’s T-Test (*** p<0.001). (E) The most sensitive positions of vein thickening in Dl^B2 / + mutants that are influenced by loss of CycG are the longitudinal vein L2 and the junction of L4 and L5 at the margin. Given is the number of flies (Fly #, Y-axis) with vein thickening at the respective position; genotypes were Dl^B2 / + (1, darker colours) and Dl^B2 cycG^HR7 / + cycG^HR7 (2, lighter colours). L2 thickness was analyzed with regard to the proximo-distal position. The proximal third of L2 (L2p) is only affected in flies lacking cycG, i.e. Dl^B2 cycG^HR7 / + cycG^HR7 animals (2), whereas the distal third (L2d) is always affected also in the Dl^B2 / + heterozygotes (1). Also shown is a comparison of L4 and L5 junctions (L4j, L5j). Note gender bias of the phenotypes: females in reddish, males in bluish colours. L2p, proximal part of L2; L2m, middle part of L2; L2d, distal part of L2; L4j, L5j, junctions of L4 and L5 with the wing margin; (n = 10).