Fig 4. Genetic interactions between cycG and Hairless mutants during wing development.

(A) Wing of a wild type fly (Ore-1); longitudinal veins L2-L5 are labelled. (B) The wing of a heterozygous H^P8/+ mutant female (Ore-1 background) shows the haplo-insufficient H phenotype characterized by a gap in the longitudinal L5 vein (encircled). (C) In the background of one mutant cycG^HR7 copy, the H wing phenotype is similar to the Ore-1 background (see also E). (D) Wing of a female fly of the genotype H^P8 cycG^HR7 / + cycG^HR7. Note complete L5 vein but gap in L4 (encircled). In general the venation phenotype H^P8 of appears enhanced in the homozygous cycG^HR7 background; the fraction of flies bearing a gap in L4 is strongly increased (see also E). (E) Graph showing the percentage of wings with vein gaps in H^P8 female flies with different genetic backgrounds. Heterozygous H^P8 females show normal venation in about 20% of the wings; in the others mostly L5 is affected (63–70%), L4 in addition much less frequently (7–15%). Rarely, L4 is solely affected (1 wing of 109 in H^P8 /+). In the homozygous cycG^HR7 background, the fraction of wings where both, L4 plus L5 are affected is increased (26–50%). Notably, the peculiar phenotype of gaps in L4 only is seen in up to 36% of the flies. Two different recombinant stocks (lines 1, 2) were analyzed, which showed a similar effect albeit with some variations. Female flies were analysed. Number of wings analyzed is given in each column.