Fig 6. Notch dependent processes during early stages of oogenesis appear unaffected in cycG mutant ovaries.

Wild type (A, C, G, I) and cycG^HR7 homozygous mutant (B, D, H, J) egg chambers and germaria (E, F) are shown; expression of each marker appears the same in both genotypes. (A, B) Expression of neur^A101-lacZ reporter line is restricted to the two polar cells (arrows) in each egg chamber. Two ovarioles are shown for each genotype. (C, D) Delta protein is most highly expressed in egg chambers between stage 5 and 7. Delta protein preferentially accumulates along the membranes: between follicle cells (closed arrow), between follicle cells and nurse cells, and between nurse cells and the oocyte (open arrow). Delta protein is also seen in the cytoplasm of the oocyte (asterisk) and the nurse cells, where it is enriched in dots (arrowhead). Posterior follicle cells overlying the oocyte display a marked downregulation of Delta expression. (E-J) Ovarioles were stained with antibodies directed against the intracellular domain of Notch. (E, F) Notch protein appears slightly enriched in the arising somatic follicle cells within the germarium (arrow). (G, H) Note accumulation of Notch protein along the membranes of follicle cells, which is highest in stage 5 to 7 egg chambers (arrow). (I, J) In a stage 10 egg chamber, Notch protein is most strongly detected in the migrating border cells (arrowhead), the centripetal cells (small arrow) and in the posterior follicle cells (open arrow).