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. 2016 Mar 10;11(3):e0151402. doi: 10.1371/journal.pone.0151402

Fig 3. WNT5A and FZD4 are highly expressed in hSVF cultures.

Fig 3

(A) RT-PCR demonstrates the robust expression of WNT5A at days 1, 3 and 5. Normalization of WNT5A expression to GAPDH indicates uniform expression of transcripts over 5 days. n = 3 donors. (B) (i.) EC were labeled with UEA1 (green), nuclei with DAPI (blue), and the entire culture with anti-Wnt5a 1° + AF594 2° antibodies (red). Robust Wnt5a expression was seen throughout the culture. Scale = 20x, 100μm. (ii.) A negative control run in parallel used only the AF594 2° antibody to rule out non-specific fluorophore binding. Scale = 20x, 100μm. (C) WNT5A receptor expression was also tested by RT-PCR (also see S3B Fig). FZD4 was consistently expressed, while densitometry shows no change in FZD4 transcripts over 5 days. n = 3 donors. NS = not significant. Bars shown as mean ± S.E.M.