Fig 6. Partial TRPC3-dependence of the hapten response.

(A) Ca²⁺ flux response by gated NK cells induced by Bourgeonal, Oxa or DNFB (red lines) in the presence of Pyr3 (10 μM) (blue lines). The bar graph depicts the mean fold change (±SEM) in Indo-1 emission (i.e. the Indo-1V/Indo-1B ratio observed at the peak response relative to that of the baseline) by the indicated compound alone (black bar) and in the presence of Pyr3 (open bar). n = 3, Statistics as compared to compound alone using t-test ** p<0.01, * p<0.05, ns not significant. (B) Ca²⁺ flux response by Jurkat cells induced by Bourgeonal, Oxa or DNFB (red lines) in the presence of Pyr3 (10 μM) (blue lines). (C) Ca²⁺ entry into TRPC3 ko (purple line) or wild type Jurkat cells (red line) induced by OKT3 antibody (2 μg/mL), Ionomycin (1 μg/mL), Bourgeonal (100 μM), Oxa (400 μM) or DNFB (250 μM). The bar graph depicts the mean fold change (±SEM) in Indo-1 emission (i.e. the Indo-1V/Indo-1B ratio observed at the peak response relative to that of the baseline) by the indicated stimulus in TRPC3 wild type (wt) (black bar) and a TRPC3 mutant clone (open bar). Data are compiled from 3–4 independent experiments (n = 3–7). Statistical significance ** p<0.01, * p<0.05, ns not significant based on unpaired t-test (p>0.05).