Figure 2. Multivalency within both the rpL5 peptide (of R-motifs) and N130 (or A tracts) is required for phase separation.
(a) Schematic representation of the NPM1 constructs used in this study; (b) Titrations of rpL5 (blue) or rpL5-RA peptide lacking the second R-motif (red), into 200 μM N130, monitored by light scattering at 340 nm; (c) Titrations of rpL5 peptide into N130 (blue) and N122 (orange).
Figure 2—figure supplement 1. A monovalent peptide with similar affinity to rpL5 for binding N122 does not phase separate with N130.
(a) ITC curve of 6R peptide titrated into N122; (b) Light scattering assays of divalent rpL5 (blue), monovalent rpL5- RA (red) and monovalent, but tighter binding 6R (aqua).