Figure 8. Phase separation by pairwise mixtures of NPM1 (N294) and molecules representing two fundamental components of the nucleolus, R-motif containing proteins (represented by R-motif peptides) and rRNA, as determined by light scattering assays.
Titration of (a) N294 into 50 μg/mL rRNA and (b) rpL5 into 40 μM N294; (c) Titrations of rRNA into 50 μM R-motif containing peptides (rpL5, GLN2, SURF6, and rpL23a). (d) Titrations of R-motif containing peptides into 50 μg/mL rRNA (as in c).