Table 2.

Binding affinities for interactions between N122 (NPM1 residues 1–122, displaying only A1) and N130 (displaying both A1 and A2) and the multivalent R-motif-containing peptides, determined using isothermal titration calorimetry (ITC), at concentrations below the critical phase separation threshold.

DOI: http://dx.doi.org/10.7554/eLife.13571.013

Peptide	N122		N130
	N (sites)	KD (μM)	N (sites)	KD (μM)
rpL5	0.63 ± 0.09	19.0 ± 2.7	2.18 ± 0.19	20.5 ± 5.0
GNL2	0.66 ± 0.04	10.2 ± 1.3	n.d.*	n.d.*
SURF6	0.55 ± 0.06	9.2 ± 0.5	1.4 ± 0.02	19.0 ± 0.8
rpL23a	n.d.#	n.d.#	n.d.*	n.d.*
rpL5-RA	1.0 ± 0.06	50.9 ± 3.18	0.84 ± 0.07	140.1 ± 9.29

^* Not determined due to multiple, unresolved binding events.

^# Heat change was too weak for accurate data analysis.

Average values from a minimum of three independent experiments are reported ± SD.