Table 4.
NMR-derived dissociation constant (KD) values determined by monitoring chemical shift perturbations of individual N130 nitrogen backbone resonances while titrating the rpL5 peptide.
| Residue | Structural context | KD (μM) |
|---|---|---|
| Met5 | N-terminus | 385 ± 108 |
| Asp6 | N-terminus | 116 ± 25 |
| Met7 | N-terminus | 142 ± 30 |
| Ser10 | N-terminus | 56 ± 13 |
| Leu12 | N-terminus | 10 ± 5 |
| Arg13 | N-terminus | 58 ± 13 |
| Gln15 | Core | 36 ± 10 |
| Tyr17 | Core | 19 ± 9 |
| Leu18 | Core | 205 ± 51 |
| Val33 | Core | 1041 ± 649 |
| Asp36 | A1 tract | 329 ± 93 |
| Glu37 | A1 tract | 1549 ± 1168 |
| Glu39 | A1 tract | 115 ± 37 |
| His40 | Core | 123 ± 23 |
| Leu42 | Core | 24 ± 9 |
| Ser43 | Core | 166 ± 38 |
| Ala64 | Core | 1227 ± 562 |
| Asn66 | Core | 1530 ± 958 |
| Tyr67 | Core | 245 ± 58 |
| Glu68 | Core | 147 ± 29 |
| Val74 | Core | 53 ± 14 |
| Phe92 | Core | 14 ± 6 |
| Glu93 | Core | 329 ± 78 |
| Ile94 | Core | 73 ± 17 |
| Thr95 | Core | 34 ± 9 |
| Leu116 | Core | 101 ± 24 |
| Val117 | Core | 61 ± 17 |
| Ala118 | Core | 35 ± 9 |
| Glu120 | A2 tract | 68 ± 16 |
| Glu121 | A2 tract | 101 ± 18 |
| Asp122 | A2 tract | 279 ± 76 |
| Ala123 | A2 tract | 182 ± 50 |
| Glu124 | A2 tract | 614 ± 410 |
| Glu126 | A2 tract | 576 ± 471 |
| Asp127 | A2 tract | 2162 ± 1612 |
| Glu130 | A2 tract | 4258 ± 1986 |