Table 5.

Kelch13 propeller domain mutations in different Plasmodium species. Here we report amino acid allele differences in a multiple sequence alignments of kelch13 homologues for seven species of Plasmodium parasites for which high-quality sequence data were available: P. falciparum (Pf), P. reichenowi (Pr), P. vivax (Pv), P. knowlesi (Pk), P. yoelii (Py), P. berghei (Pb), and P. chabaudi (Pc). The species formed three groups by similarity: Laverania (Pf, Pr), primate Plasmodia (Pv, Pk) and rodent Plasmodia (Py, Pb and Pc). An allele shared by all members of two different groups was identified as a putative ancestral allele. The table shows, for each position where at least one species exhibits a difference from the others: the amino acid position in the Pf kelch13 sequence; the putative ancestral amino acid allele; the alleles in the various species (columns with heading listing multiple species show mutations common to those species); and a substitution score of the mutation, based on a CCF53P62 substitution matrix (see Materials and methods). All substitution scores are ≥0, denoting conservative substitutions.

DOI: http://dx.doi.org/10.7554/eLife.08714.014

Pf Position	Ancestral Allele	Pf,Pr	Pv,Pk	Pk	Py,Pb,Pc	Py,Pb	Pb	Pc	Substitution Score
434	F	-	-	-	-	-	-	Y	2
447	C	-	-	-	-	S	-	-	1
448	I	-	M	-	-	-	-	-	2
517	T	V	-	-	-	-	-	-	0
519	F	Y	-	-	-	-	-	-	2
520	V	-	-	-	L	-	-	-	0
534	V	-	-	-	I	-	-	-	2
550	S	-	-	C	-	-	-	-	1
566	V	-	-	-	I	-	-	-	2
568	V	-	I	-	-	-	-	-	2
578	A	-	S	-	-	-	-	-	0
584	D	-	-	E	-	-	-	-	1
590	I	-	-	-	-	V	-	-	2
593	T	-	-	-	A	-	-	-	0
605	D	E	-	-	-	-	-	-	1
613	Q	-	-	-	-	N	-	K	0
648	D	-	-	-	E	-	-	-	1
666	V	-	-	-	I	-	-	-	2
676	A	-	-	-	T	-	-	-	0
691	D	E	-	-	-	-	-	-	1
708	I	L	-	-	-	-	-	-	0
711	S	-	-	-	-	-	P	-	0
723	I	-	-	-	V	-	-	-	2