Table 1.
Sequences of constructs and protein buffer composition.
| Construct | Protein sequence | Final buffer |
|---|---|---|
| OMP100 |
(GCN4-pII)N-IQNVDVR
STENAAR SRANEQK IAENKKA IENKADKAD VEKNRAD IAANSRA IATFRSSSQN IAALTTK-(GCN4pII)c-KLHHHHHH |
20 mM Tris pH 7.5, 400 mM NaCl, 5% Glycerol |
| Tcar0761 |
(GCN4-N16V)N-ITLMQAN
–––MATKDD LARMATKDD IANMATKDD IANMATKDD IAKLDVK IENLNTK-(GCN4-N16V)c-GSGHHHHHH |
20 mM MOPS pH 7.2, 500 mM NaCl, 5% Glycerol, 2 M Urea |
| T6 | (6xH-TEV)-(GCN4-N16V)N-MATKDD-(GCN4-N16V)c | 20 mM HEPES pH 7.4, 50 mM NaCl, 5% Glycerol, 1 M Urea |
| T9 | (6xH-TEV)-(GCN4-N16V)N-MATKDDIAN-(GCN4-N16V)c | 20 mM HEPES pH 7.4, 50 mM NaCl, 5% Glycerol, 1 M Urea |
| A6 | (6xH-TEV)-(GCN4-N16V)N-IENKAD-(GCN4-N16V)c | 20 mM HEPES pH 7.4, 50 mM NaCl, 5% Glycerol, 1 M Urea |
| A7 | (6xH-TEV)-(GCN4-N16V)N-IENKKAD-(GCN4-N16V)c | 20 mM HEPES pH 7.4, 50 mM NaCl, 5% Glycerol, 1 M Urea |
| A9 | (6xH-TEV)-(GCN4-N16V)N-IENKADKAD-(GCN4-N16V)c | 20 mM HEPES pH 7.4, 50 mM NaCl, 5% Glycerol, 1 M Urea |
| A9b | (6xH-TEV)-(GCN4-N16V)N-IANKEDKAD-(GCN4-N16V)c | 20 mM HEPES pH 7.5, 50 mM NaCl, 10% Glycerol, 1 M Urea |
(GCN4-pII)N MKQIEDKIEEILSKIYHIENEIARIKKL.
(GCN4-pII)C MKQIEDKIEEILSKIYHIENEIARIKKLI.
(GCN4-N16V)N MKQLEMKVEELLSKVYHLENEVARLKKL.
(GCN4 N16V)C MKQLEWKVEELLSKVYHLENEVARLKKLV.
(6xH-TEV) MKHHHHHHPMSDYDIPTTENLYFQGH.