Table 4.

Crystallization and cryo condition.

DOI: http://dx.doi.org/10.7554/eLife.11861.014

Structure	Protein solution & concentration	Reservoir solution (RS)	Cryo solution
OMP 100	20 mM Tris pH 7.5, 150 mM NaCl, 3% (v/v) Glycerol, 3 mg/ml protein	0.1 M tri-Sodium citrate pH 5.5, 2% (v/v) Dioxane 15% (w/v) PEG 10,000	RS + 15% (v/v) PEG 400
A6	20 mM HEPES pH 7.2, 50 mM NaCl, 2% (v/v) Glycerol, 1 M Urea, 15 mg/ml protein	95 mM tri-Sodium citrate pH 5.6, 19% (v/v) Isopropanol, 19% (w/v) PEG 4000, 5% (v/v) Glycerol	-
A7	20 mM HEPES pH 7.3, 50 mM NaCl, 1 M Urea, 15 mg/ml protein	0.1 M Citric acid pH 3.5, 3 M NaCl	-
A9	20 mM HEPES pH 7.2, 50 mM NaCl, 2% (v/v) Glycerol, 1,5 M Urea, 17 mg/ml protein	1.6 M tri-Sodium citrate pH 6.5	-
A9b black	50 mM HEPES, 50 mM NaCl, 1 M Urea, 7.5 mg/ml protein	2.4 M Sodium malonate pH 5.0	-
A9b grey	50 mM HEPES, 50 mM NaCl, 1 M Urea, 7.5 mg/ml protein	0.2 M Sodium citrate, 0.1 M Bis Tris propane pH 6.5, 20% (w/v) PEG 3350	-
T6	20 mM HEPES pH 7.2, 50 mM NaCl, 1 M Urea, 13 mg/ml protein	0.2 M CaCl2, 0.1 M HEPES pH 7.5, 30% (w/v) PEG 4000	-
T96	20 mM HEPES pH 7.2, 50 mM NaCl, 2% (v/v) Glycerol, 1.5 M Urea, 15 mg/ml protein	0.2 M Ammonium phosphate, 0.1 M TRIS pH 8.5, 50% (v/v) MPD	-
T99	„	0.1 M Citric acid pH 5.0, 20% (v/v) Isopropanol	RS + 1 M Urea +25% Glycerol
Tcar 0761	20 mM MOPS pH 7.2, 400 mM NaCl, 5% (v/v) Glycerol, 1.5 M Urea, 7 mg/ml protein	0.1 M tri-Sodium citrate pH 4.0, 30% (v/v) MPD	-