Reagent	Type	Manufacturer	Catalog #	Comments
293T cells	Cells	ATCC	CRL-3216	Original source unspecified
Dulbecco’s modified Eagle’s medium (DMEM)	Media	Invitrogen	11965118	Original unspecified
Pen/Strep	Reagent	Fisher	15140-122	Original unspecified
FBS	Reagent	Hyclone	SH30071.03	Replaces FBS from CellGro
pcDNA-IDH2WT	Plasmid	Generated in Protocol 1
pcDNA-IDH2R172K	Plasmid	Generated in Protocol 1
Lipofectamine 2000	Reagent	Invitrogen	11668027
Methanol	Reagent	Fisher	A452SK-4	Original unspecified
Refrigerated centrifuge	Equipment	Labnet International, Inc	PrismR	Original unspecified
Nitrogen gas	Reagent	Generated in lab		Original unspecified
AG-1 X8 100-200 anion exchange column	Reagent	Bio-Rad	731-6211	Poly-Prep Columns, AG 1-X8, chloride form
HCl	Reagent	Fisher	SA56-1	Original unspecified
N-methyl-N-tert- butyldimethylsily trifluoroacetamide (MTBSTFA; Regis)	Reagent	Regis	1-270243-200
Gas Chromatograph with an HP-5MS capillary column and Mass selective detector	Equipment	Agilent 7890A with 7693 Autosampler
Cold trap concentrator	Equiptment	Labconco Centrivap
R(-)-2-HG	Reagent	Sigma-Aldrich	H8378-100MG	Original unspecified