Reagent	Type	Manufacturer	Catalog #	Comments
HCT116 WT and DICEREx5 cells	Cells	Horizon Discovery	HD R02-019
siGLO RISC-free siRNA siGLO	siRNA	Dharmacon	D-001600-01-05
siGenome siRNA for nontargeting control 2	siRNA	Dharmacon	D-001210-02-05	Catalog # communicated by original authors
siGenome siRNA for SERINC1	siRNA	Dharmacon	M-010725-00-0005	Catalog # communicated by original authors
siGenome siRNA for VAPA	siRNA	Dharmacon	M-021382-01-0005	Catalog # communicated by original authors
siGenome siRNA for CNOT6L	siRNA	Dharmacon	M-016411-01-0005	Catalog # communicated by original authors
siGenome siRNA for PTEN	siRNA	Dharmacon	M-003023-02-0005	Catalog # communicated by original authors
Dulbecco's Modified Eagle's Medium (DMEM)	Cell Culture Reagent	Invitrogen	10313-039	Catalog # communicated by original authors
Fetal Bovine Serum (FBS)	Cell Culture Reagent	Invitrogen	10438-026	Catalog # communicated by original authors
Penicillin/Streptomycin	Cell Culture Reagent	Life Technologies	15140-163	Communicated by original authors
Glutamine	Cell Culture Reagent	Life Technologies	25030-081	Communicated by original authors
Trypsin	Transfection Reagent	Life Technologies	15400-054	Communicated by original authors
Dharmafect 1	Transfection Reagent	Thermo Fisher Scientific	T200104	Communicated by original authors
TRIzol reagent	qPCR reagent	Life Technologies	15596026	Communicated by original authors
RNeasy kit	qPCR reagent	Qiagen	74104	Communicated by original authors
High Capacity cDNA Archive kit	qPCR reagent	Life Technologies	4368814	Communicated by original authors
TaqMan probe PTEN	qPCR probes	Life Technologies	Hs02621230_s1
TaqMan probe CNOT6L	qPCR probes	Life Technologies	Hs00375913_m1
TaqMan probe VAPA	qPCR probes	Life Technologies	Hs00427749_m1
TaqMan probe SERINC1	qPCR probes	Life Technologies	Hs00380375_m1
TaqMan control probe ß-ACTIN	qPCR probes	Life Technologies	Hs00969077_m1	Communicated by original authors
TaqMan Fast Advanced Master Mix	qPCR reagent	Life Technologies	4444964	Communicated by original authors
StepOne Plus Real-Time PCR system	Equipment	Applied Biosystems		Replaces LightCycler 480 System
Nanodrop 2000c Spectrometer	Equipment	Thermo Scientific
PBS	Western Reagent	Life Technologies	14190250	Communicated by original authors
Lysis Buffer	Western Reagent	RIPA lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 5mM EDTA supplemented with protease inhibitors
Protease inhibitors	Western Reagent	Roche Diagnostics	11873580001	Communicated by original authors
Bradford Assay	Western Reagent	Bio-Rad		Catalog # communicated by original authors
Bis-Tris acrylamide NuPAGE gels 4–15% Mini-PROTEAN TGX Precast Protein Gels	Western Reagent	Biorad	456–1084	Replaces NuPage gels from Life Technologies (communicated by original authors)
Tris-Glycine SDS PAGE buffer (10x)	Western Reagent	National Diagostic	EC-870-4L	Replaces MOPS buffer from Invitrogen
Nitrocellulose membranes	Western Reagent	Thermo Fisher Scientific	45004006	Catalog # communicated by original authors
10xTBS buffer	Western Reagent	Biorad	170–6435	Replaces NuPage buffer from Invitrogen
Methanol	Reagent	Pharmco	339000ACSCSGL	Communicated by original authors
Mouse anti-HSP90 monoclonal antibody (90kDa)	Antibody	Becton Dickinson	61041	Catalog # communicated by original authors
Rabbit anti-PTEN monoclonal antibody (54kDa)	Antibody	Cell Signaling	9559	Catalog # communicated by original authors
Anti-mouse HRP-conjugated secondary antibody	Antibody	Abcam	Ab6728	Original not specified
Amersham ECL Western Blotting Detection Kit	Western Blot Reagent	Amersham	RPN 2108	Replaces ECL from Applied Biological Materials
X-ray Film (Hyblot CL, 8x10 inch)	Western Blot Reagent	Denville	E3018	Original not specified
Spectrophotometer	Equipment	Beckman Coulter	Spectra max M2	Replaces Beckman Model DU-800 (communicated by original authors)