Reagent | Type | Manufacturer | Catalog # | Comments |
---|---|---|---|---|
DU145 human prostate cancer cells | Cells | ATCC | HTB-81 | |
HCT116 WT and DICEREx5 cells | Cells | Horizon Discovery | HD R02-019 | |
siGLO RISC-free siRNA siGLO | siRNA | Dharmacon | D-001600-01-05 | |
siGenome siRNA for nontargeting control 2 |
siRNA | Dharmacon | D-001210-02-05 | Catalog # communicated by original authors |
siGenome siRNA for VAPA | siRNA | Dharmacon | M-021382-01-0005 | Catalog # communicated by original authors |
siGenome siRNA for CNOT6L | siRNA | Dharmacon | M-016411-01-0005 | Catalog # communicated by original authors |
siGenome siRNA for PTEN | siRNA | Dharmacon | M-003023-02-0005 | Catalog # communicated by original authors |
Dulbecco's Modified Eagle's Medium (DMEM) |
Cell Culture Reagent | Invitrogen | 10313-039 | Catalog # communicated by original authors |
Fetal Bovine Serum (FBS) | Cell Culture Reagent | Invitrogen | 10438-026 | Catalog # communicated by original authors |
Penicillin/Streptomycin | Cell Culture Reagent | Life Technologies | 15140-163 | Communicated by original authors |
Glutamine | Cell Culture Reagent | Life Technologies | 25030-081 | Communicated by original authors |
Trypsin | Transfection Reagent | Life Technologies | 15400-054 | Communicated by original authors |
Dharmafect 1 | Transfection Reagent | Thermo Fisher Scientific | T200104 | Communicated by original authors |
TRIzol reagent | qPCR reagent | Life Technologies | 15596026 | Communicated by original authors |
RNeasy kit | qPCR reagent | Qiagen | 74104 | Communicated by original authors |
High Capacity cDNA Archive kit | qPCR reagent | Life Technologies | 4368814 | Communicated by original authors |
TaqMan probe PTEN | qPCR probes | Life Technologies | Hs02621230_s1 | |
TaqMan probe CNOT6L | qPCR probes | Life Technologies | Hs00375913_m1 | |
TaqMan probe VAPA | qPCR probes | Life Technologies | Hs00427749_m1 | |
TaqMan control probe ß-ACTIN | qPCR probes | Life Technologies | Hs00969077_m1 | Additional control |
TaqMan Fast Advanced Master Mix | qPCR reagent | Life Technologies | 4444964 | Communicated by original authors |
StepOne Plus Real-Time PCR system | Equipment | Applied Biosystems | Replaces LightCycler 480 System | |
Nanodrop 2000C Spectrometer | Equipment | Thermo Scientific | ||
PBS | Western Reagent | Life Technologies | 14190250 | Communicated by original authors |
Formalin | Fixative | Sigma Aldrich | HT501128-4l | Communicated by original authors |
Crystal Violet | Stain | Sigma Aldrich | C-3886 | Communicated by original authors |
10% acetic acid | Solubilization reagent | Thermo Fisher Scientific | A38212 | Communicated by original authors |
BioTek Synergy HT Multi-mode Microplate Reader |
Equipment | BioTek Instrument | Replaces Beckman Coulter Model DU-800 (communicated by original authors) |