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. 2016 Mar 1;5:e12470. doi: 10.7554/eLife.12470
Reagent Type Manufacturer Catalog # Comments
DU145 human prostate cancer cells Cells ATCC HTB-81
HCT116 WT and DICEREx5 cells Cells Horizon Discovery HD R02-019
siGLO RISC-free siRNA siGLO siRNA Dharmacon D-001600-01-05
siGenome siRNA for
nontargeting control 2
siRNA Dharmacon D-001210-02-05 Catalog # communicated
by original authors
siGenome siRNA for VAPA siRNA Dharmacon M-021382-01-0005 Catalog # communicated
by original authors
siGenome siRNA for CNOT6L siRNA Dharmacon M-016411-01-0005 Catalog # communicated
by original authors
siGenome siRNA for PTEN siRNA Dharmacon M-003023-02-0005 Catalog # communicated
by original authors
Dulbecco's Modified Eagle's
Medium (DMEM)
Cell Culture Reagent Invitrogen 10313-039 Catalog # communicated
by original authors
Fetal Bovine Serum (FBS) Cell Culture Reagent Invitrogen 10438-026 Catalog # communicated
by original authors
Penicillin/Streptomycin Cell Culture Reagent Life Technologies 15140-163 Communicated by
original authors
Glutamine Cell Culture Reagent Life Technologies 25030-081 Communicated by
original authors
Trypsin Transfection Reagent Life Technologies 15400-054 Communicated by
original authors
Dharmafect 1 Transfection Reagent Thermo Fisher Scientific T200104 Communicated by
original authors
TRIzol reagent qPCR reagent Life Technologies 15596026 Communicated by
original authors
RNeasy kit qPCR reagent Qiagen 74104 Communicated by
original authors
High Capacity cDNA Archive kit qPCR reagent Life Technologies 4368814 Communicated by
original authors
TaqMan probe PTEN qPCR probes Life Technologies Hs02621230_s1
TaqMan probe CNOT6L qPCR probes Life Technologies Hs00375913_m1
TaqMan probe VAPA qPCR probes Life Technologies Hs00427749_m1
TaqMan control probe ß-ACTIN qPCR probes Life Technologies Hs00969077_m1 Additional control
TaqMan Fast Advanced Master Mix qPCR reagent Life Technologies 4444964 Communicated by original authors
StepOne Plus Real-Time PCR system Equipment Applied Biosystems Replaces LightCycler 480 System
Nanodrop 2000C Spectrometer Equipment Thermo Scientific
PBS Western Reagent Life Technologies 14190250 Communicated by
original authors
Formalin Fixative Sigma Aldrich HT501128-4l Communicated by
original authors
Crystal Violet Stain Sigma Aldrich C-3886 Communicated by
original authors
10% acetic acid Solubilization reagent Thermo Fisher Scientific A38212 Communicated by
original authors
BioTek Synergy HT
Multi-mode Microplate Reader
Equipment BioTek Instrument Replaces Beckman Coulter Model DU-800 (communicated by original authors)