Table 1.
Proteins that are significantly reduced in the secretome upon Cre recombinase induced deletion of ADAM10. The table contains the 42 most reduced proteins upon ADAM10 deletion. Indicated are the names of the proteins, the gene name, number of unique peptides, topology, the mean of the ratio between neurons devoid of ADAM10 and neurons expressing endogenous levels of ADAM10 of 5 biological replicates and the p-value calculated with a two-sided, heteroscedastic t-test based on the intensity ratios for the control and the ADAM10 deletion condition. Protein names of previously published ADAM10 substrates are highlighted in italic bold. (MEAN) Mean value of all 5 biological replicates, (SEM) Standard error of the mean. (Peptides) Number of peptides identified for every protein. Gene symbols of proteins validated with immunoblot are marked bold.
| Protein Name | Gene Symbol | Peptides | Topology | MEAN (A10KO/CON) |
TTEST |
|---|---|---|---|---|---|
| Matrix metalloproteinase-17 | Mmp17 | 3 | GPI | 0,05 | 1,77E-03 |
| Podocalyxin-like protein 2 | Podxl2 | 3 | Type-I | 0,05 | 3,13E-03 |
| Cadherin-10 | Cdh10 | 4 | Type-I | 0,05 | 1,15E-03 |
| Protocadherin-8 | Pcdh8 | 16 | Type-I | 0,06 | 1,06E-03 |
| SLIT and NTRK-like protein 1 | Slitrk1 | 4 | Type-I | 0,08 | 8,52E-03 |
| Sulfhydryl oxidase 2 | Qsox2 | 3 | Type-I | 0,08 | 7,47E-03 |
| Fractalkine | Cx3cl1 | 2 | Type-I | 0,09 | 6,08E-03 |
| Cell adhesion molecule 4 | Cadm4 | 5 | Type-I | 0,09 | 1,11E-03 |
| Poliovirus receptor-related protein 1 | Pvrl1 | 3 | Type-I | 0,10 | 1,05E-02 |
| Leucine-rich repeat and immunoglobulin-like domain-containing nogo | Lingo2 | 2 | Type-I | 0,11 | 8,21E-04 |
| Semaphorin-7A | Sema7a | 8 | GPI | 0,12 | 1,24E-02 |
| Receptor-type tyrosine-protein phosphatase U | Ptpru | 6 | Type-I | 0,12 | 1,35E-02 |
| Latrophilin-1 | Lphn1 | 7 | Type-I | 0,13 | 2,81E-02 |
| Neural cell adhesion molecule 1 | Ncam1 | 16 | Type-I/GPI | 0,14 | 1,24E-03 |
| Leucine-rich repeat-containing protein 4B | Lrrc4b | 16 | Type-I | 0,16 | 7,03E-04 |
| Cell adhesion molecule 3 | Cadm3 | 5 | Type-I | 0,16 | 2,30E-02 |
| Low-density lipoprotein receptor | Ldlr | 13 | Type-I | 0,16 | 1,04E-02 |
| Neuroligin-1 | Nlgn1 | 5 | Type-I | 0,17 | 8,12E-04 |
| Receptor-type tyrosine-protein phosphatase gamma | Ptprg | 4 | Type-I | 0,17 | 4,03E-03 |
| H(+)/Cl(-) exchange transporter 3 | Clcn3 | 2 | Polytopic | 0,19 | 7,17E-03 |
| Cadherin-6 | Cdh6 | 5 | Type-I | 0,19 | 7,46E-03 |
| VPS10 domain-containing receptor SorCS1 | Sorcs1 | 3 | Type-I | 0,23 | 2,96E-03 |
| Neuronal growth regulator 1 | Negr1 | 3 | GPI | 0,23 | 2,37E-02 |
| Vesicular integral-membrane protein VIP36 | Lman2 | 3 | Type-I | 0,25 | 2,86E-02 |
| Receptor-type tyrosine-protein phosphatase kappa | Ptprk | 12 | Type-I | 0,26 | 1,12E-03 |
| SLIT and NTRK-like protein 3 | Slitrk3 | 3 | Type-I | 0,26 | 1,84E-02 |
| Cell adhesion molecule 1 | Cadm1 | 6 | Type-I | 0,26 | 3,85E-02 |
| Transmembrane protein 132A | Tmem132a | 2 | Type-I | 0,26 | 1,52E-03 |
| Immunoglobulin superfamily member 3 | Igsf3 | 25 | Type-I | 0,27 | 8,04E-03 |
| Protein tweety homolog 1 | Ttyh1 | 5 | Type-I | 0,28 | 1,79E-02 |
| Torsin-1A-interacting protein 1 | Tor1aip1 | 5 | Polytopic | 0,29 | 2,62E-02 |
| Protocadherin 9 | Pcdh9 | 2 | Type-II | 0,29 | 3,15E-02 |
| Protocadherin-20 | Pcdh20 | 2 | Type-I | 0,30 | 3,28E-02 |
| Plexin-B2 | Plxnb2 | 4 | Type-I | 0,30 | 3,64E-02 |
| Semaphorin-4B | Sema4b | 4 | Type-I | 0,32 | 3,98E-02 |
| Neuronal cell adhesion molecule | Nrcam | 4 | Type-I | 0,33 | 4,91E-03 |
| RGM domain family member B | Rgmb | 3 | Type-I | 0,35 | 1,06E-02 |
| Chondroitin sulfate synthase 2 | Chpf | 2 | Type-I | 0,35 | 7,63E-03 |
| Neogenin | Neo1 | 27 | Type-I | 0,35 | 3,27E-03 |
| Collagen alpha-1(XI) chain | Col11a1 | 4 | GPI | 0,36 | 4,53E-02 |
| Beta-1,3-galactosyltransferase 6 | B3galt6 | 2 | Type-II | 0,38 | 4,85E-02 |
| Neuroligin-3 | Nlgn3 | 28 | Type-I | 0,38 | 2,97E-04 |