Table 2.
Proteins that are significantly changed in the cellular glycoproteome upon Cre recombinase induced deletion of ADAM10. The table contains all significantly changed proteins upon ADAM10 deletion. Indicated are the names of the proteins, the gene name, number of unique peptides, the mean of the ratio between neurons devoid of ADAM10 and neurons expressing endogenous levels of ADAM10 of 4 biological replicates and the p-value calculated with a two-sided, heteroscedastic t-test based on the relative label-free quantification ratios (LFQ) for the control and the ADAM10 deletion condition. Gene Symbols of previously published ADAM10 substrates are highlighted in bold. (MEAN) Average change between A10 deleted (A10KO) and Control (CON) cells of 4 biological replicates, (SEM) Standard error of the mean. (Peptides) Number of Peptides identified for every protein.
| Protein Name | Gene Symbol | Peptides | Topology | MEAN (A10KO/CON) |
TTEST |
|---|---|---|---|---|---|
| Isoform APP695 of Amyloid beta A4 protein | App | 15 | Type I | 3,71 | 1,30E-02 |
| Fractalkine | Cx3cl1 | 6 | Type I | 2,91 | 3,00E-03 |
| Protein Ptprz1 | Ptprz1 | 25 | Type I | 2,41 | 1,66E-03 |
| Receptor-type tyrosine-protein phosphatase U | Ptpru | 7 | Type I | 2,10 | 7,82E-03 |
| Immunoglobulin superfamily containing leucine-rich repeat protein 2 | Islr2 | 16 | Type I | 1,89 | 6,18E-03 |
| Semaphorin-4B | Sema4b | 4 | Type I | 1,81 | 2,36E-02 |
| Contactin-associated protein-like 4 | Cntnap4 | 17 | Type I | 1,65 | 3,55E-02 |
| Fibronectin leucine rich transmembrane protein 3 | Flrt3 | 16 | Type I | 1,64 | 5,05E-02 |
| Netrin receptor UNC5D | Unc5d | 5 | Type I | 1,61 | 4,66E-02 |
| Neural cell adhesion molecule L1 | L1cam | 33 | Type I | 1,61 | 1,12E-02 |
| Voltage-dependent L-type calcium channel subunit beta-1 | Cacnb1 | 9 | Type I | 1,60 | 2,78E-02 |
| Phospholipase D3 | Pld3 | 3 | Type II | 1,60 | 4,49E-02 |
| Melanoma inhibitory activity protein 3 | Mia3 | 23 | Type I | 1,55 | 2,04E-02 |
| Receptor-type tyrosine-protein phosphatase kappa | Ptprk | 12 | Type I | 1,53 | 2,41E-02 |
| Tetraspanin-7 | Tspan7 | 5 | Polytopic | 1,49 | 1,63E-02 |
| Protein Pcdhgb6 | Pcdhgb6 | 9 | Type I | 1,49 | 9,66E-03 |
| Serine/threonine-protein kinase LMTK3 | Lmtk3 | 19 | Type I | 1,47 | 2,53E-02 |
| Protocadherin-8 | Pcdh8 | 32 | Type I | 1,46 | 2,73E-03 |
| Protein Pcdha9 | Pcdha9 | 12 | Type I | 1,43 | 3,90E-02 |
| Glutamate receptor 1 | Gria1 | 21 | Type I | 1,42 | 1,00E-02 |
| Tenascin-R | Tnr | 17 | Secreted | 1,40 | 4,69E-03 |
| Neurocan core protein | Ncan | 6 | Secreted | 1,35 | 7,81E-03 |
| Protein disulfide-isomerase A6 | Pdia6 | 7 | Secreted | 1,34 | 5,00E-02 |
| Contactin-2 | Cntn2 | 18 | GPI | 1,30 | 1,87E-02 |
| Receptor-type tyrosine-protein phosphatase F (Fragment) | Ptprf | 19 | Type I | 1,30 | 4,74E-02 |
| Reticulon-4 | Rtn4 | 39 | Polytopic | 1,28 | 2,94E-04 |
| Isoform 2 of Seizure protein 6 | Sez6 | 6 | Type I | 1,27 | 3,76E-02 |
| Large neutral amino acids transporter small subunit 1 | Slc7a5 | 9 | Polytopic | 1,25 | 1,50E-03 |
| Cell adhesion molecule 4 | Cadm4 | 10 | Type I | 1,23 | 3,02E-02 |
| Transferrin receptor protein 1 | Tfrc | 22 | Type II | 1,18 | 3,52E-02 |
| Cadherin EGF LAG seven-pass G-type receptor 3 | Celsr3 | 35 | Type I | 1,16 | 1,37E-02 |
| Protocadherin Fat 4 | Fat4 | 48 | Type I | 1,12 | 3,05E-02 |
| Sodium/potassium-transporting ATPase subunit alpha-3 | Atp1a3 | 62 | Polytopic | 0,95 | 4,09E-02 |
| Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 | Atp2a2 | 52 | Polytopic | 0,86 | 2,14E-02 |
| Slc8a1 protein | Slc8a1 | 24 | Polytopic | 0,85 | 3,18E-02 |
| Synaptic vesicle glycoprotein 2A | Sv2a | 15 | Polytopic | 0,85 | 6,99E-03 |
| Solute carrier organic anion transporter family member 3A1 | Slco3a1 | 4 | Polytopic | 0,75 | 8,95E-03 |