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. 2016 Mar 1;5:e12052. doi: 10.7554/eLife.12052

Figure 2. A low dose of cocaine selectively reduces p-eIF2α in the VTA of adolescent mice.

(a–b) A low dose of cocaine (5 mg/kg) reduced p-eIF2α in the VTA of adolescent (p<0.05, n=5 per group, t8=3.029), but not adult mice (p=0.329, n=3 per group, t4=1.110). A higher dose of cocaine (10 mg/kg) was needed to reduce p-eIF2α in VTA of adult mice (p<0.001, n=6 per group, t10=4.640). (c) Schematic of mTORC1- and eIF4E-mediated translation. In abilescnt mice, a low dose of cocaine (5 mg/kg) did not significantly alter phosphorylation of S6K at Thr-389 (d), 4E-BP1 at Thr-37 and Thr-46 (e) and eIF4E at Ser209 (f). Western blots are shown on top and quantification for each phospho-protein/total-protein is shown at the bottom (n=3/3 saline/cocaine; S6K, p=0.3467, t4=01.066a; 4E-BP1, p=0.5031, t4=0.7351; eIF4E, p=0.5669, t4=0.6233). Plots are mean ± s.e.m.

DOI: http://dx.doi.org/10.7554/eLife.12052.008

Figure 2.

Figure 2—figure supplement 1. Doses of cocaine which lower p-eIF2α in the VTA have no effect in nucleus accumbens (NAc). .

Figure 2—figure supplement 1.

(a) Scheme of the experimental procedure (b) A low dose of cocaine (5mg/kg) or a higher dose of cocaine (10 mg/kg) had no effect on p-eIF2 in the NAc of adolescent (p=0.678, n=3 per group, t4=0.4) or adult mice (p=0.18, n=3 per group, t4=1.6), respectively. Plots are mean ± s.e.m.
DOI: http://dx.doi.org/10.7554/eLife.12052.009