Extended Data Figure 1.

MCD, CDAA and HF diet induce NASH and promote HCC.

a, Representative imagines of Oil Red O staining of MYC-ON mice fed MCD or CTR. Black bar = 100 μm. b, Serum ALT levels analysis. Mean ± SEM; n=4, *p<0.05, one-way ANOVA. c-e, The effect of CDAA diet on tumor development in MYC transgenic mice. Experimental set-up, representative liver images and liver surface tumor counts are shown. Black bar=10 mm. Mean ± SEM; n= 6 for CDAA and n=5 mice for CTR, p=0.0345, Student’s t test. f-i, The effect of CDAA and HF diet on liver carcinogenesis in DEN-injected C57BL/6 mice. Experimental set-up, representative tumor-free H&E stainings, macroscopic liver images and surface tumor counts are shown. Black bar=100 μm. Mean ± SEM; n=13 for CTR, n=9 for HF, n=10 for CDAA, *p<0.05, one-way ANOVA.