Extended Data Table 1.
Fatty Acid Composition of Hepatic Lipids.
| Fatty Acid | ON-CTR | ON-MCD | OFF-CTR | OFF-MCD |
|---|---|---|---|---|
| C12:0 | 17.2 ± 4.2 | 16.7 ± 2.1 | 10.3 ± 2.2 | 18.6 ± 2.6 |
| C14:0 | 143.9 ± 8.1 | 204.8 ± 21.1 | 195.2 ± 34.4 | 237.6 ± 25.7 |
| C15:0 | 86.9 ± 6.1 | 62.2 ± 6.1 | 77.9 ± 4.2 | 53.1 ± 4.0 |
| C16:0 | 10986.0 ± 703.0 | 7576.4 ± 486.5a | 11325.9 ± 517.8 | 8274.1 ± 543.9b |
| C16:1 | 467.2 ± 49.0 | 88.5 ± 7.7 | 430.9 ± 52.6 | 169.2 ± 17.7 |
| C17:0 | 216.1 ± 18.4 | 199.0 ± 17.2 | 249.8 ± 17.0 | 171.7 ± 12.9 |
| C17:1 | 0.5 ± 0.2 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| C18:0 | 6861.1 ± 520.0 | 7228.8 ± 463.1 | 8335.6 ± 287.9 | 7700.4 ± 557.0 |
| C18:1 | 1498.2 ± 126.0 | 1432.8 ± 108.1 | 1545.8 ± 102.3 | 1773.6 ± 111.2 |
| C18:2 | 4664.8 ± 464.5 | 6492.8 ± 356.1a | 4516.3 ± 582.9 | 7584.1 ± 462.3b |
| C18:3N3 | 30.0 ± 3.8 | 183.5 ± 23.3 | 28.3 ± 4.0 | 187.7 ± 20.1 |
| C18:3N6 | 71.0 ± 8.0 | 37.8 ± 2.4 | 65.1 ± 8.0 | 48.5 ± 2.8 |
| C18:4 | 2.3 ± 0.4 | 3.7 ± 0.5 | 1.4 ± 0.3 | 4.1 ± 0.4 |
| C20:0 | 80.8 ± 6.8 | 29.0 ± 2.4 | 16.4 ± 1.1 | 13.2 ± 1.3 |
| C20:1 | 2.5 ± 0.3 | 3.1 ± 0.4 | 1.1 ± 0.2 | 1.8 ± 0.2 |
| C20:2 | 119.7 ± 8.7 | 183.0 ± 14.3 | 108.3 ± 4.3 | 158.3 ± 16.7 |
| C20:3N3 | 53.0 ± 4.0 | 38.0 ± 5.6 | 53.8 ± 5.7 | 24.1 ± 3.8 |
| C20:3N6 | 973.8 ± 28.6 | 592.2 ± 75.3 | 709.6 ± 33.2 | 529.5 ± 58.0 |
| C20:3N9 | 13.5 ± 1.2 | 3.4 ± 0.3 | 10.0 ± 0.8 | 2.2 ± 0.3 |
| C20:4 | 10503.7 ± 751.6 | 10949.5 ± 799.5 | 14287.7 ± 619.5 | 10801.1 ± 1218.5b |
| C20:5 | 118.6 ± 9.7 | 25.0 ± 2.1 | 103.3 ± 10.3 | 28.5 ± 2.0 |
| C22:0 | 19.8 ± 1.6 | 6.5 ± 0.7 | 7.8 ± 1.0 | 3.2 ± 0.2 |
| C22:1 | 20.3 ± 1.6 | 5.5 ± 0.5 | 6.1 ± 0.3 | 2.2 ± 0.3 |
| C22:2 | 7.8 ± 0.9 | 2.5 ± 0.3 | 0.2 ± 0.1 | 0.0 ± 0.0 |
| C22:3 | 2.4 ± 0.4 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| C22:4 | 140.1 ± 8.3 | 419.9 ± 42.2 | 136.7 ± 4.3 | 296.4 ± 37.5 |
| C22:5N3 | 454.8 ± 29.2 | 997.8 ± 76.6 | 523.1 ± 23.4 | 706.5 ± 114.3 |
| C22:5N6 | 336.1 ± 29.8 | 80.0 ± 6.8 | 206.1 ± 7.6 | 87.7 ± 11.4 |
| C22:6 | 9237.6 ± 687.1 | 5207.0 ± 388.0a | 10704.6 ± 426.9 | 6414.9 ± 638.9b |
| C23:0 | 1.2 ± 0.2 | 0.2 ± 0.1 | 0.2 ± 0.1 | 0.0 ± 0.0 |
| C24:0 | 6.5 ± 0.4 | 2.9 ± 0.5 | 3.3 ± 0.4 | 1.8 ± 0.1 |
| C24:1 | 1.1 ± 0.1 | 0.4 ± 0.1 | 0.2 ± 0.0 | 0.0 ± 0.0 |
| Total | 47313.0 ± 3047.1 | 41742.3 ± 2471.9 | 53664.5 ± 1953.3 | 45295.3 ± 3554.2 |
Mouse livers from MYC-on or MYC-OFF mice receiving 4 weeks of MCD or control diet treatment were subjected to total fatty acid profiling by GC/MS as described in Material and Methods. Results (in pmol/mg tissue) are expressed as mean± SEM (n=6/group,
a
p<0.05 for ON-CTR vs ON-MCD,
b
p<0.05 for OFF-CTR vs OFF-MCD, two-way ANOVA)