Table 6.

Comparison of recently published protocols for MGE induction (Pur: Purmorphamine, SB: SB431542, LDN: LDN193189, BF: BMPR1A–Fc, XV: XAV939, SAG: smoothened agonist).

Author	Initial culture	Neural induction	Ventralization	Rostralization	%Nkx2.1+ (%)
Liu et al.	Sphere	–	d11–25 1.5 µM Pur	–	90
Germain et al.	Adherent	500 ng/ml noggin	d6–16 500 ng/ml SHH	–	46.70
Nicholas et al.	Sphere	10 µM SB 1.5 µg/ml BF	d0–14 1 µg/ml DKK1 d0–35 1–2 µM Pur	–	90.80
Maroof et al.	Adherent	10 µM SB 100 nM LDN	d0–10 2 µM XV d10–18 1µM Pur 5 nM SHH	–	~50
Kim et al.	Sphere	10 µM SB 100 nM LDN	d0–14 5µM IWP2 d0–21 100 nM SAG	100 ng/ml FGF8	81.80