Table 2.

Fatty acid composition of total polar lipids and different phospholipids and phospholipid compositions of S. pombe grown at either 30 or 36 °C

	Growth temp. (°C)	TPL	PC	PE	PI	PS	PG + CL
16:0	30	14.8 ± 0.4	7.4 ± 0.4	11.2 ± 0.3	28.5 ± 1.0	24.4 ± 0.2	13.9 ± 0.8
	36	18.4 ± 1.0	11.6 ± 1.6	16.3 ± 0.1	30.2 ± 0.8	27.2 ± 0.1	17.5 ± 1.0
16:1	30	1.2 ± 0.1	0.5 ± 0.8	2.6 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	1.0 ± 1.4
	36	1.3 ± 0.0	0.6 ± 0.8	3.1 ± 0.8	0.3 ± 0.5	0.0 ± 0.0	1.1 ± 1.6
18:0	30	5.6 ± 0.5	4.6 ± 0.7	2.2 ± 0.4	7.9 ± 1.0	7.0 ± 0.7	12.3 ± 0.6
	36	7.3 ± 0.4	6.3 ± 1.5	3.6 ± 1.1	8.9 ± 0.1	7.8 ± 0.3	14.0 ± 0.4
18:1	30	77.9 ± 0.1	87.4 ± 0.3	84.0 ± 0.7	63.6 ± 2.0	68.6 ± 0.5	58.1 ± 3.6
	36	72.4 ± 0.8	81.5 ± 2.3	77.0 ± 0.3	60.5 ± 1.1	64.9 ± 0.2	49.6 ± 2.7
30:0	30	0.5 ± 0.1	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	14.7 ± 5.2
	36	0.6 ± 0.3	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	17.8 ± 4.9
DBI/sat	30	3.8 ± 0.0	7.3 ± 0.7	6.5 ± 0.4	1.7 ± 0.2	2.2 ± 0.0	1.5 ± 0.3
	36	2.9 ± 0.1*	4.6 ± 0.5*	4.0 ± 0.3*	1.6 ± 0.0	1.9 ± 0.0*	1.0 ± 0.2
Lipid class (%) #	30	n/a	38.7 ± 2.7	20.4 ± 1.8	25.6 ± 3.1	7.4 ± 1.3	4.4 ± 1.0
	36	n/a	40.9 ± 5.2	16.4 ± 1.6	27.0 ± 4.2	7.0 ± 0.6	4.9 ± 0.8

S. pombe lipids were separated on TLC and analyzed by GC-MS (see “Methods”). FA data are expressed as weight % of total FAs present in each lipid classes and presented as means ± SD (n = 3). DBI/sat, double bond index/saturated FAs, was calculated as the ratio of the sum of the weight % of unsaturated FAs multiplied by the number of double bonds for each FA and the sum of the weight % of saturated fatty acids. The lipid composition was determined by GC-MS of the FA of each PL, using an internal standard and is given as a % of the total PL

n/a not applicable

*Values significantly different from the corresponding data at 30 °C (p < 0.05); ^#The difference between the sum of polar lipid class percentages and 100 % represents the sum of minor components (3–4 %) not given in the table