Figure 4.
Blue-light mediated changes in phot1 and NPH3 phosphorylation status. Immunoblot analysis of total protein extracts from 3-day-old etiolated WT and phot1 phot2 (p1p2) mutant seedlings maintained in darkness (D) or irradiated with 20 μmol m−2 s−1 of over-head blue light for 15 min (L). Protein extracts were probed with anti-phot1 (upper panel), anti-NPH3 antibodies (middle panel) and anti-UGPase antibody as a loading control (lower panel). The dashed lines indicate the highest mobility edge.