Table 2.
Genus- and species-specific primers of Butyrivibrio
| Primer region | Species and Primer sequence | Product size (bp) | Annealing temp (°C) |
|---|---|---|---|
| 16s rRNA |
ButUnivF: 5′-CTATCAGCAGGGAAGAAAG-3′ ButUnivR1: 5′-GTTAGCGACGGCACTGA-3′ |
420 | 53.5 |
| 16s rRNA |
ButUnivF: 5′-CTATCAGCAGGGAAGAAAG-3′ ButUnivR2: 5′-CCGTCAATTCCTTTGAGTTTC-3′ |
482 | 54 |
| 16s rRNA |
Species-specific (
Butyrivibrio fibriosolvens
)
BfibF: 5′-ACACACCGCCCGTCACA-3′ BfibR: 5′-TCCTTACGGTTGGGTCACAGA-3′ |
220 | 55 |
| 16s rRNA |
Species-specific (
Butyrivibrio proteoclasticus
)
ButproF: 5′-ACTCCTACGGGAGGCAG-3′ ButproR: 5′-CTGAATGCCTATGGCACCCAA-3′ |
505 | 52 |
| 16s rRNA |
Species-specific (
Butyrivibrio hungatei
)
ButHungF: 5′-CCGCATAAAACAGCAGAGTCGCAT-3′ ButHungR: 5′-TAGCACGTGTAGCCCAAG-3′ |
1150 | 58 |