Figure 2.

Increased cell proliferation in the murine DG after chronic administration with PPX, but not after ROP treatment. (A) Proliferation paradigm. 3-month-old C57BL/6 received either i.p. PPX, ROP or PBS for 14 days. At day 14, animals were i.p. injected with BrdU (100 mg/kg) twice to label proliferating cells. Mice were perfused at day 15. (B) Quantification of BrdU⁺ cells in the DG revealed an increased density of proliferating cells after PPX compared to ROP and PBS. Representative stainings of BrdU⁺ cells in the DG of PBS- (C), PPX- (D), and ROP-injected animals (E). Error bars represent mean ± SEM. One-way ANOVA followed by Tukey's post-hoc test, ^*p < 0.05. Scale bar: 50 μm (C–E).